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Detection of DNA Hybridyzation with Impedance Amplifying Labels

K-S Ma, H. Zhou, A. Fischetti, J. Zoval and M. Madou
University of California, Irvine, US

Keywords: DNA, streptavidin, alkaline phosphatase, electrochemical impedance spectroscopy, impedance

Abstract:
Traditionally used nucleic acid analysis detection platforms rely on fluorescent or radioactive labels. Optical techniques require large and expensive equipment such as automated colorimetric and fluorimetric instruments that greatly increase the cost of the whole set-up. Working with radioactive labels is an even less attractive option. Electrical measurements of DNA hybridization are expected to involve less complicated and less expensive instrumentation and feature detection limits similar to the traditional optical methods. The diagnostic technique we introduce here will enable low-cost molecular diagnostics methods to become a reality and broaden the extent of the nucleic acid analysis applications.

Nanotech 2004 Conference Technical Program Abstract

 
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