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Effect of Substratum Morphology on Cell Behavior

W-T Su, I-M Chu, J-Y Yang and C-D Lin
National Nano Device Laboratories, TW

Keywords: contact guidance, stromal, cytoskeletal, plasma etching, microfabrication

Abstract:
Contact guidance induced by the surface topography of the substrata influences the direction of cells with artificial material. Mouse bone marrow stromal fibroblast was examined on grooved substrata of varying dimension (0.3£gm~1.2£gm groove width, 0.5£gm depth and 0.5£gm ridge). It was found that the repeat space had an effect in determining cell alignment. Measurement of cell alignment and examination by scanning electron microscopy showed the fibroblast interacted grooved substrata in monomodal responses and alignment to the direction of the grating.

Nanotech 2004 Conference Technical Program Abstract

 
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