Two-Regime Hollow PDMS Abbe Prism for u-TAS Applications

A. Llobera, R. Wilke and S. Büttgenbach
Institut für Mikrotechnik, DE

Keywords: micro total analysis systems, laser induced fluorescence, optical detection

Abstract:
A new robust and compact system, which comprises a PDMS-based hollow Abbe prism and microlenses, together with self-alignment channels for optical fibre positioning, is presented. This optical detection method has two different regimes for signal transduction, namely absorption and refractive index shift. µM concentrations of fluorescein are detected with this system using standard equipment (SLED and photodetector). The results clearly show the regions in which each physical effect is significant.

Nanotech 2004 Conference Technical Program Abstract