Nano Science and Technology InstituteNano Science and Technology Institute
Nano Science and Technology Institute 2004 NSTI Nanotechnology Conference & Trade Show
Nanotech 2004
BioNano 2004
Program
Topics & Tracks
Sunday
Monday
Tuesday
Wednesday
Thursday
Index of Authors
Keynotes
Awards
Tutorials
Business & Investment
2004 Sub Sections
Sponsors
Exhibitors
Venue 2004
Proceedings
Organization
Press Room
Purchase CD/Proceedings
NSTI Events
Subscribe
Site Map
 
Nanotech Proceedings
Nanotechnology Proceedings
Supporting Organizations
Nanotech Supporting Organizations
Media Sponsors
Nanotech Media Sponsors
Event Contact
696 San Ramon Valley Blvd., Ste. 423
Danville, CA 94526
Ph: (925) 353-5004
Fx: (925) 886-8461
E-mail:
 
 

Development of a Slug-Flow PCR Chip with Minimum Heating Cycle Times

S. Hardt, D. Dadic, F. Doffing, K.S. Drese, G. Münchow and O. Sörensen
Institute of Microtechnology Mainz, DE

Keywords: microfluidics, PCR, heat transfer, slug flow

Abstract:
A microfluidic PCR device is presented capable of rapid temperature ramping and handling of sample volumes in the microliter and submicroliter range. The PCR chip comprises a straight micro channel in which a sample slug is periodically moved over three temperature zones. As part of the sample preparation a method for metering of the sample volume was developed. The PCR chip and the chips for sample preparation and fluidic actuation were fabricated by ultra-precision milling in polymer substrates. Computer simulations suggest that the sample slugs are heated or cooled on a time scale of some ten milliseconds when transported to a different temperature zone. The fluidic actuation based on a ferrofluid transducer is capable of positioning the sample volumes with a high accuracy after a large number of cycles. The design developed should be ideally suited for fast PCR of small sample amounts in a highly parallel manner.

Nanotech 2004 Conference Technical Program Abstract

 
Sponsors
Nanotech Sponsors
News Headlines
NSTI Online Community
 
 

© Nano Science and Technology Institute, all rights reserved.
Terms of use | Privacy policy | Contact