Optically Defined patterning and Compositional Engineering of Supported Bilayer Lipid Membranes Using Deep UV Photochemistry

A.N. Parikh, C.K. Yee and M.L. Amweg
Univeristy of California Davis, US

Keywords:

Abstract:
We describe a simple, generic method for micro-patterning supported bilayer membranes simply using a physical mask and ozone-generating UV light. In particular, spatially-directed exposure of sBLMs to UV light in the 184-257 nm range, in conjunction with ensuing production of activated oxygen and ozone, affords stable microsocopic sBLM patterns by removal of exposed lipids (and proteins) leaving behind disjointed membrane patches at optically defined locations on the substrate surface. Following slight lateral expansion, the residual membrane architecture stabilizes within the confined corrals presumably by self-sealing at the membrane-water interface through the hydrophobic effect.. Refunctionalization of the pattern-edges using different lipids, proteins, and antibodies provides new opportunities to create bifunctional micro-arrays displaying alternating arrays of functionality in single chips. The patterning and refunctionalization methods are applicable to a wide range of planar and non-planar bilayer configurations.

NSTI Nanotech 2003 Conference Technical Program Abstract