Mimicking Immune Processes by Positioning Antibodies on Surfaces - A Nanoengineering Approach
G-Y Liu, N. Amro, V. Komanicky and M. Liu
University of California at Davis, US
Keywords: immune processes, positioning antibodies, surfaces, nanoengineering
Antibody-antigen interaction is one of the most fundamental biospecific interactions. While the antibody-antigen monovalent and divalent bindings are relatively well-known in solution phase, controlling the valency on surfaces has not been attempted. Using scanning probe nanolithography, antigens such as small ligand groups or proteins can be positioned on surfaces with nanometer precision. Subsequent reactions with specific antibodies result in the formation of antibody-antigen complexes on surfaces. The monovalent and divalent interactions could occur, depending upon the position of the antigen nanostructures, and the reaction conditions. Examples and detailed results of this nanoengineering approach will be included in the presentation.
NSTI Nanotech 2003 Conference Technical Program Abstract