Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in Microsystems

K.Ohtaguchi and K.Asami
Tokyo Institute of Technology, JP

Keywords: DNase I, Pichia pastoris, Mathematical model, excretion system

Abstract:
A mathematical model for the dynamics of micro-scale production of recombinant human DNase I is formulated. In experiments extracellular production of human DNase I gene product from methylotrophic yeast Pichia pastoris GS115 has been achieved. A micro-scale production of recombinant human DNase I on a plug flow micro-scale reactor in steady-state was modeled mathematically. Simulation result suggests that the space time at which the maximum productivity of the gene product occurs is 37h. Micro-scale unit to separate endonuclease from cells was also designed using liquid-liquid extraction. Partition coefficient was considered in the models.

NSTI Nanotech 2003 Conference Technical Program Abstract